Strand ReadPairAntiSenseRate

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One part of the RNA-Seq QC is the strand enrichment rate. In this section, take one fly dataset (SRR070272) as one example, where 99.86% of ReadPairs are mapped to the forward strand of the transcriptome.

RnaSeq FlyDataStrand.png

In this table, the Strand_ReadPairAntiSense denotes total count of Read pairs mapped to the reverse strand of transcriptome reference. The Strand_ReadPairAntiSenseRate is calculated by

Equation strandReadPairAntiSense Rate.png

RNA-Seq QC for the strand-specific dataset (SRR070272 is available from both GEO and our tutorial);