Ngs DnaSeqPipeline.pdf

From Array Suite Wiki

Jump to: navigation, search

Contents

DNA-Seq Pipeline

DNA-Seq Pipeline module allows users to finish the whole DNA-Seq analysis in a single click. Based on user's selection, it will run the following pipeline:

DNASeqPP1.png

After alignment, it will load BAM files once and finish all analyses.

The pipeline module is particularly better than individual modules when enable cloud based analysis with less file transfers and saving EC2 machine setup time.

General

DNASeqPP2.png

Input/Output

Accepted file formats include FASTQ, FASTA, QSEQ, and AUTO, BAM

If user is using BAM files as input, the module will use AddGenomeMappedDnaSeqReads to add alignment file as NgsData directly for all downstream analysis. The pipeline has been tested briefly for external BAM files generated by other aligners (outside of Omicsoft). We are recommending users starting from raw (fastq/fasta) files. There is a ConvertNgsFiles module for user to convert BAM back to fastq.gz.

Basic

In the Basic section, the user has a number of options:

  • The user can choose whether this is a paired end sequencing analysis, and if so, the reads will automatically be paired using a numbering logic (e.g. _1, _2 or .1, .2).
  • Choose a Genome and Gene model The OmicSoft genome models for Human do not have the recommended decoy sequences. Consider building your own model or using another tool for the alignment step with human data
  • File format in Gzip, Bzip2 or None
  • Replace existing BAM files in the output folder or skip the alignment step for samples already having BAM files in the output folder
  • Number of thread for each alignment and number of jobs/samples running in parallel.
  • Additional alignment settings can be added using Oscript syntax. User can get all possible alignment parameter options from MapDnaSeqReads.

Reporting

Check box to run each individual pipeline module.

Users are required to specify output name and folder.

Results

There are multiple result data objects in Omicsoft Project:

  • NgsData object for DNA-Seq alignment
  • Table report for raw data QC, alignment report, alignment QC, and mutation detection report.

DNASeqPP3.png

All result files in the output folders

DNASeqPP4.png

OmicScript

DnaSeqPipeline