From Array Suite Wiki
Install Array Studio on Windows
Windows prerequisites: Microsoft .NET 3.5
From Internet Explorer (IE) browser, open the following link:
For first time users, see How to activate your Array Studio.
Install Array Studio on Mac
Please follow these instructions to install Array Studio on Mac.
Install Array Server On Linux
Required Linux packages
Make sure you have some basic packages installed:
yum install gcc gcc-c++ bison pkgconfig libtool libstdc++-devel \ glib2-devel gettext make freetype-devel fontconfig-devel \ libXft-devel libpng-devel libjpeg-devel libtiff-devel giflib-devel \ ghostscript-devel libexif-devel libX11-devel
The user can either use an sqlite-based database for Array Server, or Oracle. If using Sqlite, version 3.6.4 or higher is required. It's recommended to install Sqlite with the same prefix as that used to install mono.
Sqlite can be downloaded from: sqlite download. The tar.gz file is recommended for installation.
wget -c http://www.sqlite.org/sqlite-autoconf-3071401.tar.gz tar zxvf sqlite-autoconf-3071401.tar.gz sudo mv sqlite-autoconf-3071401 sqlite cd sqlite ./configure --prefix=/opt/sqlite make make install sqlite3 --version
The libgdiplus package must be installed (either using yum, apt-get, or installing from source at libgdiplus).
cd /opt wget http://download.mono-project.com/sources/libgdiplus/libgdiplus-2.10.tar.bz2 tar jxvf libgdiplus-2.10.tar.bz2 cd /opt/libgdiplus-2.10 ./configure --prefix=/opt/libgdiplus-2.10 make make install
If installing from source, you may need to add the "/libgdiplusPrefix/lib" to the shared library search paths, and check to make sure libgdiplus library is on the shared library search paths. To check, type
$ ldconfig -p | grep libgdiplus libgdiplus.so (libc6,x86-64) => /opt/libgdiplus-2.10/lib/libgdiplus.so
Here the libgdiplus is installed at "/opt/libgdiplus" and has been set correctly. If not, one way to add it to the shared library path is by doing this (with root privilege),
echo "/opt/libgdiplus/lib" > /etc/ld.so.conf.d/libgdiplus.conf ldconfig
If Unix admin does want to add libgdiplus in shared library, he/she can modify the following config file:
/MonoPrefix/etc/mono/config: Add the following line at the end of the file before </configuration>
<dllmap dll="gdiplus.dll" target="/opt/libgdiplus-2.10/lib/libgdiplus.so"/>
If installing from yum, it may be necessary to additionally install the following:
yum install libungif libungif-devel
Make sure the ulimit for "max user processes" and "open files" are set to the max value: 65536. You can check the values by typing: ulimit -a.
$ ulimit -a core file size (blocks, -c) 0 data seg size (kbytes, -d) unlimited scheduling priority (-e) 0 file size (blocks, -f) unlimited pending signals (-i) 515184 max locked memory (kbytes, -l) 64 max memory size (kbytes, -m) unlimited open files (-n) 65536 pipe size (512 bytes, -p) 8 POSIX message queues (bytes, -q) 819200 real-time priority (-r) 0 stack size (kbytes, -s) 10240 cpu time (seconds, -t) unlimited max user processes (-u) 65536 virtual memory (kbytes, -v) unlimited file locks (-x) unlimited
Modify ulimit in two config files following ulimit setup wiki.
We recommended to use mono 2.10.9 for NGS alignment in Linux.
- Download Mono 2.10.9
The bz2 file can be saved to a temporary location, e.g. ~/temp/
- Extract and modify certificate if necessary
tar jxvf mono-2.10.9.tar.bz2 cd /opt/mono-2.10.9
For mono 2.10.9, it is recommended to modify X509Certificate to the latest standard.
- Compile and install. On the command line, type
cd /opt/mono-2.10.9 ./configure --prefix=/opt/mono-2.10.9 --with-large-heap=yes make make install
- The location of mono installed is set by the option "--prefix" in the configure step, which can be changed to another location.
- The option --with-large-heap=yes is to enable support for GC heaps larger than 3gb, which is required for NGS alignment, as well as some Array Server functions)
Double check mono installation and version
ls /opt/mono-2.10.9/bin/mono* -all /opt/mono-2.10.9/bin/mono --version /opt/mono-2.10.9/bin/mono-sgen --version
Install Array Server
Download Array Server
Assume we would like to install the Array Server under the directory /opt
- On command line, type
mkdir /opt/arrayserver cd /opt/arrayserver wget -c http://omicsoft.com/software_update/OmicsoftUpdater.exe
- Next, create an empty file named ArrayServerLinuxBeta.exe by typing
- Then, type
cd /opt/arrayserver /opt/mono/bin/mono ./OmicsoftUpdater.exe
Download configuration template
cd /opt/arrayserver wget http://omicsoft.com/software/ArrayServer/ArrayServerConfigTemplate.zip unzip ArrayServerConfigTemplate.zip
Admin will have to modify three important configuration files based on downloaded template:
- ArrayServer.cfg is the key configuration file. A few important options are :
- Port, Port2 and Port3 define the port number for data communication between ArrayStudio client and ArrayServer. Make sure to open these ports on firewall.
- DataPortBegin and DataPortEnd define the port range for Threaded Ftp data transfer. Make sure to open the port range on firewall.
- BaseDirectory: this will be the working directory of the array server, storing all the raw and possessed data. Depending on the projects, it can take huge amount of disk space.
- OmicsoftDirectory: This directory can sit locally or on a network drive. All the reference genomes, gene models, Affymetrix CDF files, log files, etc. are stored in this folder.
- TempDirectory: This should be a local directory (i.e. NOT a network drive) for fast read and write access. It can take twice the size of an unzipped fastq file in some NGS tasks (we would suggest use a drive with at least 100GB storage).
- The Folder section defines additional local or network folders monitored and available for access by array server users.
- For a full list of options, see ArrayServer.cfg for more details.
- For master-analytic server setup, please read Master Server and Analytic Server for more details.
- default.template: it defines project level meta data, such as requiring project title, and list organism. Admin can customize the project meta data based on this template file and even enforce controlled vocabulary. For first time setup, the default values we prepared should be good to go. Admin can further customized based on the understanding of default.template wiki article.
- sample.template: it defines sample level meta data, such as requiring users to fill organism and tissue for each samples in sample registration. Admin can customize the sample meta based on this template file and even enforce controlled vocabulary. For first time setup, the default values we prepared should be good to go. Admin can further customize based on the understanding of sample.template wiki article.
Start Running ArrayServer
- For first time admin, you can use Typical way to update/restart ArrayServer. Basically, it uses mono-sgen to run
- For advanced admin, server can be maintained using crontab and shell script such as Run-omicsoft.sh
Once the ArrayServer is ready and running, each user can connect to ArrayServer using the server tab in their ArrayStudio client:
Install Array Server On Windows
Test Your Installation
Please watch the video tutorial for server analysis basics and test the ArrayServer connectivity.
- Getting Started With Server Analysis
- Running Array Server in Windows
- Master Server and Analytic Server
- Get started with Oshell